Changes in the expression of TRPV4 and TRPM8 channels in the colon of rats with 6-OHDA-induced Parkinson’s disease

Parkinson’s disease (PD) is neurodegenerative disease, which accompanied by degeneration of dopaminergic neurons in subtantia nigra. Non-motor symptoms, particular, disorders the gastrointestinal (GI) tract are observed 20-80% patients some 15-20 years before clinically diagnosed PD and not a least important feature pathogenesis. The transient receptor potential (TRP) channels expressed throughout GI tract, where they play an role taste, thermoregulation, pain, mucosal function homeostasis, control interstitial motility etc. aim this study was to investigate contribution TRPV4 TRPM8 motor colon rats with PD, incduced injection 12 μg 6-hydroxydopamine (6-OHDA). studies were performed on 4th week 7th month after induction randomly divided into: I group – sham-lesioned rats, 4 μl 0.9% NaCl, autopsy weeks (n = 5); II 6-OHDA-PD 6-OHDA, III 7 months 4); IV 5). We evaluated body weight transit time, cecum index immunohistochemical identification tyrosine hydroxylase (TH) -positive cells, TRPV4, expression rat’s colon. showed that experiment, time doubles over time; 6-OHDA increased 57%; number TH-positive cells decreased 2-fold, while ion downregulated upregulated 6-OHDA-PD. It concluded may be considered pharmacological targets progression pathology.